Journal: Veterinary Sciences

Article Title: Clinical Spatial Distribution of Aquaporin-1 in Camel Cornea Using Assistive AI Applications

doi: 10.3390/vetsci13050425

Figure Lengend Snippet: Representative photomicrographs of the camel cornea. Panels (C1, MD1, MV1) show H&E-stained sections illustrating epithelial thickness in the central (C), middle dorsal (MD), and middle ventral (MV) regions (scale bar: 100 µm). Panels (C2, MD2, MV2) depict the stromal layer and Descemet’s membrane in the same regions following H&E staining. Panels (C3, MD3, MV3) demonstrate AQP1 immunoreactivity within the corneal epithelium and keratocytes of the anterior stroma, with variable staining intensity across regions (black arrows; scale bar: 50 µm). Panels (C4, MD4, MV4) show AQP1 localization in the posterior stroma and endothelium, where immunostaining is primarily confined to keratocytes and endothelial cells (black arrows; scale bar: 50 µm).

Article Snippet: Subsequently, the sections were incubated for 60 min with a rabbit polyclonal anti-human AQP1 primary antibody (1:1000; catalog no. GB11310, Servicebio, Woburn city, MA, USA).

Techniques: Staining, Membrane, Immunostaining

Journal: Veterinary Sciences

Article Title: Clinical Spatial Distribution of Aquaporin-1 in Camel Cornea Using Assistive AI Applications

doi: 10.3390/vetsci13050425

Figure Lengend Snippet: Representative photomicrographs of the camel cornea from the middle nasal (MN), middle temporal (MT), and peripheral dorsal (PD) regions. Panels (MN1, MT1, PD1) show H&E-stained sections illustrating epithelial thickness in the corresponding regions. In addition, vascular structures are visible in the peripheral dorsal region (PD2), likely associated with the limbal area (black arrows; scale bar: 100 µm). Panels (MN2, MT2, PD2) demonstrate the stromal layer and Descemet’s membrane in these regions following H&E staining. Panels (MN3, MT3, PD3) reveal AQP1 immunoreactivity within the corneal epithelium and keratocytes of the anterior stroma, with regional variation in staining intensity (black arrows; scale bar: 50 µm). Panels (MN4, MT4, PD4) illustrate AQP1 localization in the posterior stroma and endothelium, where staining is predominantly confined to keratocytes and endothelial cells (black arrows; scale bar: 50 µm).

Article Snippet: Subsequently, the sections were incubated for 60 min with a rabbit polyclonal anti-human AQP1 primary antibody (1:1000; catalog no. GB11310, Servicebio, Woburn city, MA, USA).

Techniques: Staining, Membrane

Journal: Veterinary Sciences

Article Title: Clinical Spatial Distribution of Aquaporin-1 in Camel Cornea Using Assistive AI Applications

doi: 10.3390/vetsci13050425

Figure Lengend Snippet: Representative photomicrographs of the camel cornea from the peripheral ventral (PV), peripheral nasal (PN), and peripheral temporal (PT) regions. Panels (PV1, PN1, PT1) show H&E-stained sections illustrating epithelial thickness in the respective regions (scale bar: 100 µm). Vascular structures are evident in the peripheral areas (PV2, PN2, PT2), likely corresponding to extensions of the limbal vasculature (black arrows). Panels (PV2, PN2, PT2) further demonstrate the stromal layer and Descemet’s membrane following H&E staining. Panels (PV3, PN3, PT3) display AQP1 immunoreactivity within the corneal epithelium and keratocytes of the anterior stroma, with noticeable regional differences in staining intensity (black arrows; scale bar: 50 µm). The strongest epithelial expression of AQP1 was observed in the peripheral nasal region (PN3), highlighted by white circles. Panels (PV4, PN4, PT4) illustrate AQP1 localization in the posterior stroma and endothelium, where staining is primarily confined to keratocytes and endothelial cells (black arrows; scale bar: 50 µm). Additionally, panel (PT5) shows the presence of brown melanin granules within the peripheral temporal region (black arrows; scale bar: 50 µm).

Article Snippet: Subsequently, the sections were incubated for 60 min with a rabbit polyclonal anti-human AQP1 primary antibody (1:1000; catalog no. GB11310, Servicebio, Woburn city, MA, USA).

Techniques: Staining, Membrane, Expressing

Journal: Veterinary Sciences

Article Title: Clinical Spatial Distribution of Aquaporin-1 in Camel Cornea Using Assistive AI Applications

doi: 10.3390/vetsci13050425

Figure Lengend Snippet: Immunohistochemical localization of AQP1 in camel corneal epithelium across different cellular layers, including superficial, intermediate (polyhedral), and basal cells. The columns represent the relative expression levels of AQP1 in the following corneal regions according to Area Fraction (%): central (C), middle dorsal (MD), middle nasal (MN), middle temporal (MT), middle ventral (MV), peripheral dorsal (PD), peripheral nasal (PN), peripheral temporal (PT), and peripheral ventral (PV). Data are presented as Mean ± SD (n = 6). Different superscript letters above bars indicate statistically significant differences between groups (One-way ANOVA followed by Tukey’s post hoc test, p < 0.05).

Article Snippet: Subsequently, the sections were incubated for 60 min with a rabbit polyclonal anti-human AQP1 primary antibody (1:1000; catalog no. GB11310, Servicebio, Woburn city, MA, USA).

Techniques: Immunohistochemical staining, Expressing

Journal: Veterinary Sciences

Article Title: Clinical Spatial Distribution of Aquaporin-1 in Camel Cornea Using Assistive AI Applications

doi: 10.3390/vetsci13050425

Figure Lengend Snippet: Immunohistochemical distribution of AQP1 in the camel cornea, including the anterior and posterior stromal regions as well as the endothelium. The columns illustrate the relative expression levels of AQP1 across different corneal regions according to Area Fraction (%): central (C), middle dorsal (MD), middle nasal (MN), middle temporal (MT), middle ventral (MV), peripheral dorsal (PD), peripheral nasal (PN), peripheral temporal (PT), and peripheral ventral (PV). Data are presented as Mean ± SD (n = 6). Different superscript letters above bars indicate statistically significant differences between groups (One-way ANOVA followed by Tukey’s post hoc test, p < 0.05).

Article Snippet: Subsequently, the sections were incubated for 60 min with a rabbit polyclonal anti-human AQP1 primary antibody (1:1000; catalog no. GB11310, Servicebio, Woburn city, MA, USA).

Techniques: Immunohistochemical staining, Expressing

Journal: Veterinary Sciences

Article Title: Clinical Spatial Distribution of Aquaporin-1 in Camel Cornea Using Assistive AI Applications

doi: 10.3390/vetsci13050425

Figure Lengend Snippet: Proposed model for the spatial distribution of AQP1 water channels in the camel cornea in the three corneal layers, epithelium, stroma and endothelium. The green color shows AQP1 localization in the different corneal epithelial cell layers; superficial, polyhedral, and basal cell layers. The black color shows localization of AQP1 in keratocyte cells of stroma, while the red color clarifies the localization of AQP1 in corneal endothelium.

Article Snippet: Subsequently, the sections were incubated for 60 min with a rabbit polyclonal anti-human AQP1 primary antibody (1:1000; catalog no. GB11310, Servicebio, Woburn city, MA, USA).

Techniques:

Journal: Veterinary Sciences

Article Title: Clinical Spatial Distribution of Aquaporin-1 in Camel Cornea Using Assistive AI Applications

doi: 10.3390/vetsci13050425

Figure Lengend Snippet: Topographical map of AQP1 distribution across the nine corneal regions. The schematic represents the regional intensity of AQP1 expression in the epithelium (EPI), stroma (STR), and endothelium (EN) of the camel cornea. The AI-generated Area Fraction (AF %) data: (+) = Weak expression (AF < 2%), (++) = Moderate expression (AF = 2–4%), (+++) = Strong expression (AF = 4–6%) and (++++) = Very strong expression (AF > 6%).

Article Snippet: Subsequently, the sections were incubated for 60 min with a rabbit polyclonal anti-human AQP1 primary antibody (1:1000; catalog no. GB11310, Servicebio, Woburn city, MA, USA).

Techniques: Expressing, Generated

Journal: bioRxiv

Article Title: Proximity proteomics reveals a role for IFI16 during human coronavirus infection

doi: 10.64898/2026.04.22.720112

Figure Lengend Snippet: IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of HCoV-OC43 N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: Cells were washed twice with a permeabilization/wash buffer (0.1% saponin and 0.5% BSA in 1X PBS) and stained with a 1:10000 dilution of anti-HCoV-OC43-N rabbit polyclonal antibody (catalogue no. 40643-T62; Sino Biological) for 30 min at 4°C.

Techniques: Infection, CRISPR, Knock-Out, Single Cell, Clone Assay, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Incubation, Staining, Flow Cytometry

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Standalone methacrylated extracellular matrix for digital light processing bioprinting: a practical workflow

doi: 10.3389/fbioe.2026.1774476

Figure Lengend Snippet: 3D bioprinted dECM-MA bioink supports high-density parenchymal cell attachment, and early ECM remodeling. Human uterine stromal fibroblast cells (primary parenchymal cells of the uterine tissue, FC-0076 Lifeline Cell Technologies) were cultured in commercial media, exhibiting characteristic spindle-shaped stromal morphology (A) . These cells were immunofluorescently characterized for positive uterine and stromal markers (CD10, CD73, and Vimentin, respectively). CD31 isotype staining served as a negative control. Following expansion, cells were seeded at high density onto 3D bioprinted dECM-MA scaffolds, cultured for 16 h, and characterized by HR-SEM (B) . An overview SEM image shows a visibly high cell density on the ECM-MA after culture. Higher magnifications reveal a high-density (yellow rectangle, zoomed in) and lower density (blue rectangle, zoomed in) areas where human uterine stromal cells (yellow arrow) interact with the dECM-MA fibers (green arrow), suggesting biological interaction and constructive remodeling.

Article Snippet: Specifically, rabbit anti-human CD10 (Bioss, Cat. No. BS-0527R-20; RID: AB_10854297) and rabbit anti-human vimentin (Bioss, Cat. No. BS-0756R-20; RRID: AB_10855343) were used as primary antibodies, with a rabbit IgG isotype control (Santa Cruz Biotechnology, Cat. No. sc-8306; RRID: AB_653100).

Techniques: Cell Attachment Assay, Cell Culture, Staining, Negative Control